The Makler Counting Chamber is only 10 microns deep: 1/10th of the depth of ordinary hemocytometers, making it the shallowest of known chambers. Constructed from two pieces of optically flat glass, the upper layer serves as a cover glass, with a 1 sq. mm fine grid in the center subdivided into 100 squares of 0.1 x 0.1 mm each. Spacing is firmly secured by four quartz pins.
The Makler Counting Chamber for Rapid Semen Evaluation